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National Vaccine Program Office
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Proposal Number: | N19 |
PI Name: | Sampson, Jacquelyn S. |
PI Email: | |
PI Title: | Microbiologist |
Project Title: | Development of a novel Streptococcus pneumoniae microarray for detection of pneumococci, antibiotic sensitivity, and diagnosis of pneumococcal disease. |
Project Start: | 2003 |
Project End: | 2004 (Phases 1 and 2) |
Abstract: Diagnostic tests are problematic for the majority of disease burden attributable to S. pneumoniae, complicating the ability to evaluate the specific effect of new or investigational vaccines against noninvasive disease such as pneumonia. Culture is the gold standard but is only 15-30 percent positive. The project goal is to develop a respiratory microarray for diagnosis of pneumococcal disease and for detection, identification, and typing of S. pneumoniae including drug resistant S. pneumoniae. A microarray respiratory chip approach may provide serotype specific and antimicrobial resistance data in addition to species identification capability simultaneously, without bacterial culture, resulting in a diagnostic tool of great rapidity and sensitivity.
The study consists of 3 phases: 1) gene selection, sequence design, and construction; 2) identification of additional genes for inclusion in microarray construction; and 3) evaluation of developed array using strains grown in vitro, spiked specimens, and in vivo specimens (mouse models of infection, human carriers, pneumococcal disease patients).
Successful implementation of this project will have a significant impact on pneumococcal disease diagnosis and epidemiologic research. It will facilitate accurate determination of pneumococcal disease burden, efficient evaluation of new protein vaccines for prevention of pneumococcal disease, as well as ongoing monitoring of the impact of pneumococcal conjugate vaccine. By providing a device for more precise diagnoses, which also distinguishes serotypes and antibiotic resistance, our capacity to respond in outbreak and crisis situations will be enhanced. Finally, this pneumococcal microarray could be combined with those designed for other respiratory organisms to construct a diagnostic microarray for all human respiratory pathogens.
Institution: Centers for Disease Control and Prevention
Date: July 2003
Last revised: January 13, 2004
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